The present study investigated the performance of Pitahaya (Hylocereus spp.) peel (PP) as a low-cost biosorbent in the removal of Co(II), Cd(II), Pb(II), and Ni(II) from single and multi-component solutions. The characterization of the samples was carried out by pHpzc, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDS). Biosorption was carried out by batch experimental procedure to examine the effects of contact time, solution pH, initial concentration of metal ions, and biosorbent dosage. The results indicate that the biosorption of Pb(II), Cd(II), Co(II), and Ni(II) Pitahaya peels followed pseudo-second-order kinetics, and equilibrium adsorption followed the Langmuir model. The maximum sorption capacities of PP for the metallic species were found to be as follows: Pb (82.64 mg g⁻¹) > Cd (17.95 mg g⁻¹) > Co (6.013 mg g⁻¹) > Ni (5.322 mg g⁻¹). However, the efficiency of the biosorption change when the metallic species are mixed. The re-generation of the PP after the adsorption of the metallic species was done using 0.1 M HNO₃ solution, and the reusability of the biomass was carried out using two adsorption and desorption cycles.