Dragon Fruit Info
First report of
causing fruit rot of durian in the Philippines
Management of Pests and Diseases
Tamie C. Solpot, Christian Joseph R. Cumagun
Murray) known as the ‘king of fruits’ is widely cultivated in the tropics of Asia. In September 2019, durian fruit rot was observed in Calinan, Davao City, Mindanao, Philippines (7º10.9620’N; 125º25.0480’E). About 10–15% of fruits in a durian orchard displayed mild to severe infections. Symptoms included small brown to grey lesions up to severe rot, with aerial white mycelial on the surface of infected fruits. Fragments of infected tissues (2–3 mm
) were excised from the diseased-healthy parts of fruits, disinfected with 10% NaOCl for 2 min, rinsed twice in distilled sterile water, and incubated on V8 juice agar. The colony of isolate (DDV-F4) of the pathogen was typically petaloid. Coenocytic hyphae (6.3—11.61 μm wide) had distinctive hyphal swellings. Sporangia were globose to ovoid and measured 7.3–12.1 × 5.6–10.90 μm. Morphological characters suggested
S. Takim (Santoso et al.
). To confirm the taxonomy of the organism, molecular identification was carried out. Genomic DNA was extracted and the partial ITS region was amplified with ITS1 and ITS4 primers (White et al.
). BLAST searches in NCBI GenBank showed that the sequence of the isolate DDV-F4 (MT280219) had 99.76% similarity to
(KP183959) from Indonesia. To establish the pathogenicity of the pathogen, five healthy detached leaves and five matured fruits were inoculated with 5-mm-diam. mycelial plugs from 10-day-old axenic culture of isolate DDV-F4. Sterile agar plugs were used in the control. Two–three days after inoculation dark brown-grey circular to irregular (when they coalesced) lesions with aerial white mycelial appeared on leaves and fruits. The pathogen was successfully re-isolated from inoculated leaves and fruits.
was previously reported from Indonesia (Santoso et al.
) and Thailand (Suksiri et al.
), from the soil of durian orchards. To our knowledge, this is the first report of the pathogen on durian in the Philippines.
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